In the last post, I covered the basics of wound-healing assays and how to quantify and visualise them. This is great, but is the ability of cells to heal an artificial wound really what you want to measure? Probably not…
The wound healing assay is a fairly common technique in the Cell Biology Research toolkit. The idea is simple: grow cells to cover a surface, scratch a ‘wound’ into the cells then see how well the cells can close the wound (either by replication, migration or a combination of both).
The blood! The horror!
In this post, we’ll be using Fiji to analyse this type of assay which will give us lots of flexibility in both methodology and visualisation options.
