A lot of work we do at the CCI uses scanning confocal microscopes, which have the advantage that the operator can pick the number of pixels in X and Y that will make up the final image.
For camera-based systems this is a less simple endeavour as the array of the CCD chip is fixed. For this reason, we may want to downsample or bin our images. In this post we’ll cover a bit of theory and details on how (and why) to bin your images.
Sampling and more specifically, sampling frequency is a really important and much misunderstood concept in many fields of research. As we’ll see in this analogy-ridden post, it’s important to understand sampling in both time and space.