Tag Archives: Resolution

It’s a Stitch up!

Scale is a funny thing. To virologists, bacteria are big. To microbiologists, cells are big. To cell biologists, tissues are big. We’ll not go as far as the cosmologists, but the point is that sometimes you have to move out of your size-based comfort zone and image something BIG. Enter imaging tiling!

2015-06-stitch-00

Once you’ve acquired all of your images (36 in the example above), it’s not always clear (even in vendor software) how to put them back together. Today’s post will deal with image stitching and also cover one of the big problems you may run into along the way.

Continue reading

Resolving Disputes

Super-resolution has been receiving a lot of press in the last 6 months, largely given the 2014 Nobel prize to Betzig, Hell and Moerner “for the development of super-resolved fluorescence microscopy“.

In this post, we’re going to take a look at one way to accurately measure the size of a point emitter which is helpful when used as a metric for resolving power.

Continue reading