Tag Archives: 8bit

Know thy Image Part 2: Image Histograms

In the last post, we looked at the following image to decide if an intensity of 2000 at the tip of the arrowhead was bright or dim. Without knowing the bit depth of the image it’s impossible to tell.

Bright or not bright?

We posed that if this was a 12bit image, that’s fairly bright (2000/4096) but if it’s a 16bit image, then it’s very dim (2000/65536). But why would these two conditions look the same? The answer lies in the histograms and transfer functions…

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Know thy Image Part 1: Bit Depths

In the list of things that everyone who uses a microscope should know, this has to be near the top and yet I find a surprising number of people are either never taught it or don’t fully grasp the idea of Bit Depth. In this post and Part 2, we will deal with (almost) everything you need to know about bit depth, dynamic range and image histograms.

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