Unless your imaging facility is in a clean room (and you never touch it), from time to time, we all end up with unsightly splotches on our transmitted light images. The best fix for this is to clean the microscope but sometimes you just have to do what you can with what you’ve got.
Thankfully there’s a fairly easy way to correct it post-acquisition. Let’s Flat-field correct!
A lot of work we do at the CCI uses scanning confocal microscopes, which have the advantage that the operator can pick the number of pixels in X and Y that will make up the final image.
For camera-based systems this is a less simple endeavour as the array of the CCD chip is fixed. For this reason, we may want to downsample or bin our images. In this post we’ll cover a bit of theory and details on how (and why) to bin your images.
Whenever you’re testing a new analysis protocol or playing around with some software, it’s always handy to have some sample data to mess with. But what if you don’t yet have the data, or what if you need more, or need more specific data? In this post, we’re going to delve into the world of synthetic data by making a sample tracking data set.
One of the more annoying things about fluorescence imaging is that it’s a bit like trying to describe our location in the universe. There’s no absolute point of reference, the values are rather arbitrary and you rely heavily on relative measures (like being 1AU from our local star).
This post will demonstrate some of the problems with quantifying basic fluorescent images and use a case study to show how ratiometric imaging (among other things) can be used to solve them.
In previous posts, we’ve almost taken for granted how Fiji/ImageJ deals with multidimensional data. Whenever you have more than one image, be it a second channel all the way up to massive 5D datasets, you’re dealing with stacks.
This concept is so fundamental, I thought it deserving of it’s own post. Let’s stack ’em up.
Arguably one of the best things about doing Research using microscopes is the awesome power of the Image for getting your point across. Whether it’s for public outreach, presentation at a conference or just showing off your work at group meeting, a picture says… well you know the rest.
But what if you’re looking at a temporal phenomenon? Sure you could always use a montage but there’s nothing like a moving picture to wow your audience and get pulses racing. In this post we’ll look at a few different ways of turning your multidimensional data into movies.
In the book of things you didn’t know you can do in Fiji, I’ll bet we’re going to cover a couple here today. The Research community uses the Portable Document Format as the de facto standard for ‘reprints’ of published articles. In this post, we’ll look at how to open, modify, save and deconstruct PDF files all from the comfort of Fiji.