Monthly Archives: March 2015

Know thy Image Part 1: Bit Depths

In the list of things that everyone who uses a microscope should know, this has to be near the top and yet I find a surprising number of people are either never taught it or don’t fully grasp the idea of Bit Depth. In this post and Part 2, we will deal with (almost) everything you need to know about bit depth, dynamic range and image histograms.

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Going (peri)Nuclear

Localisation analysis is all about asking where proteins, organelles, cells or anything else are. This is, of course fairly meaningless unless you have some point of reference. In today’s post, we’re going to be looking at quantifying perinuclear localisation.

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A matter of scale (bars)

One of the most frequent questions that I get asked is how to add a scale bar to an image. While it’s good that people are adding scale bars to images for presentations, posters and papers, it’s not always done terribly well. In this post, we’ll look at two ways to do it and why you would favour one or the other

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Size (and shape) matters – especially at the nanoscale (Part 1)

With the (semi-) inaugural post for the blog, I’m going to get right down to it. In the Lévy Lab, we’re experimenting with Open Notebook Science, currently with a project involving SmartFlares.

Lab member Joan Comenge took some really nice Transmission Electron Microscopy (TEM) images of our gold nanoparticles, which look something like this:

TEM of SmartFlares (Uptake Control)

TEM of SmartFlares (Uptake Control)

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Welcome!

Hi there! I work at the Liverpool Centre for Cell Imaging at the University of Liverpool, helping users with their Image Analysis problems. Broadly, image analysis is the process of turning images into numbers. Related to this are Image Acquisition (capturing images) and Data Analysis (turning numbers into something meaningful).

WorkflowI find many of the problems I come across are really interesting, which is reason enough to share them. The other point of this blog however, is to disseminate the methods, techniques and processes that I use, in the hope that other people might find them useful and instructive (and also as a repository for me to direct people to when they have a specific problem).

I hope you enjoy the blog! I always welcome feedback in the comments section or on Twitter @dn_mason.